Authors: Philip Keith Pattemore, Lance C. Jennings
Categories: Article
Source: Pediatric Respiratory Medicine
This chapter deals with three aspects of the epidemiology of respiratory infections in common respiratory viruses, acute lower respiratory tract infection (ARI), and emerging/reemerging organisms.
Respiratory tract infections are among the most frequent diseases in early life. Many viruses are known to be associated with symptomatic respiratory tract infections, the most common being respiratory syncytial virus (RSV), influenza viruses types A and B, parainfluenza viruses, adenoviruses, and rhinoviruses. Viral infections are consistently more commonly found in younger children,1, 2 among whom viruses are the cause of as many as 90% of all lower respiratory tract infections (LRIs).^3^ Increasingly, these viruses are also being associated with a substantial burden of respiratory disease in adults, including elderly persons, and in immunocompromised persons. The advent of molecular technologies has improved laboratory detection of virus in clinical samples and extended the ability to characterize the epidemiology of respiratory virus infections, but it has also led to the identification of subtypes and genotypes of known respiratory viruses and to the discovery of novel respiratory viruses.
Conventional virologic methods, including antigen detection, serology, and culture, have clearly identified the viruses most frequently causing respiratory illnesses in children and associated with both upper respiratory tract (URI) and LRI infections. Table 31-1 shows the relative frequency of viruses identified within the Tecumseh, Michigan, community study, from 1976 to 1981.^4^
The inclusion of nucleic acid technologies (e.g., polymerase chain reaction [PCR]) for the detection of respiratory virus infections in children has substantially increased the diagnostic sensitivity for most respiratory viruses.5, 6, 7 This technology has allowed the recovery of viruses that have been difficult to detect by conventional methods.^8^ For example, PCR is three to five times as sensitive as cell culture for detecting rhinoviruses.9, 10 Figure 31-1 shows the viral identification breakdown in three recent studies of children5, 11, 12 in different countries over the winter viral season using PCR techniques. This figure illustrates the differences between studies in patient sampling and methods and the consequent differences in results. However, in all three studies, rhinovirus and RSV are the dominant viruses, with the relative proportions of each differing even between the two studies in infants (studies A and B). Mixed viral infections, particularly with rhinovirus, are common. Finally, studies find only the organisms they look for—only study C tested for bocavirus.
Figure 31-1 Three studies of virus isolation in childhood respiratory tract infection (RTI) over winter using molecular virology. Data sources and details are as A, Legg et al.^11^Denominator: 123 RTIs in a cohort of 88 infants with at least one atopic parent, followed in Southampton, UK. Specimens: nasal lavage. *Virology * individual RT-PCR for six virus groups including picornavirus (not specifically identified as rhinovirus), M. pneumoniae, and *C. pneumoniae.*B, Jennings et al.^5^Denominator: 75 children (65 under 12 months old) presenting to hospital in Christchurch, New Zealand, with acute respiratory illness suggesting lower RTI. Specimens: nasopharyngeal swabs. *Virology * PCR/RT-PCR for 11 viruses as well as immunofluorescence and viral culture. C, Arden et al.^12^ and personal communication, Ian Mackay. Denominator: 315 respiratory specimens obtained from people (1 day to 80 years old, 79% under 5 years old) presenting with RTIs to Brisbane hospitals, selected from all four seasons in 2003-2004. Specimens: not described. Virology: immunoassay, culture, and 17 PCR/RT-PCR assays.
Molecular methods have also allowed the rapid identification and characterization of previously unknown viruses causing respiratory illness. The human metapneumovirus (hMPV) was first identified in the Netherlands in 2000^13^ and shows a wide geographic distribution.14, 15 Five new human coronaviruses have been discovered,^16^ including severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV),17, 18 human coronavirus NL63 (hCoV-NL63),^19^ and human coronavirus HKU1 (hCo-HKU1).^20^ Another virus, the human bocavirus (hBoV), has also been identified in association with respiratory illness in hospitalized patients.^21^
Studies using conventional virology have detected multiple respiratory viruses in 1.8% to 15.8% of acute LRIs.^22^ While more recent studies using nucleic acid technologies have identified multiple viruses in up to 27% of hospitalized children with LRI.^5^ All of the common respiratory viruses have been identified as contributing to multiple infections. They have been recognized more frequently in younger children (≥4 years) and in hospitalized children. The presence of more than one virus may result in more severe or prolonged infection.15, 23, 24 Alternately, because young children are immunologically naïve and experience a median of 4.4^25^ to 5.5^26^ respiratory illnesses per year and because viral shedding is commonly prolonged in young children, a continuum of residual virus or viral nucleic acid may be being detected.
Both the periodicity and the seasonality of viruses that cause respiratory tract infections are well established in temperate climates3, 11, 26 (Fig. 31-2 ; also Fig. 31-1). Most viruses circu late in a community every year; however, some, especially influenza type B and the parainfluenza viruses, may cause epidemics at biennial or longer intervals.^3^ Different viruses also predominate in a community during different seasons of the year; overlap invariably occurs and the actual timing and severity of each outbreak or epidemic can vary from year to year. In tropical climates, seasonality also exists. In these regions, RSV occurs predominantly in the rainy season, whereas influenza activity occurs throughout the year with a marked peak in activity during the dry season and a lesser peak during the rainy season.27, 28 Seasons of both higher and lower temperature have been associated with these viruses in different countries.^27^ The reasons for the seasonality of respiratory virus infections and their variation by latitude are not clear. More important than the environmental conditions themselves may be their effect on behavior. Seasons when people spend more time indoors, in closer contact with other people (colloquially called “closed-in season” in some regions), will favor more rapid person-to-person transmission.
Figure 31-2 Monthly rates per 100 children of defined virus-specific episodes in the Finnish Otitis Media Cohort Study (red lines). This was a population cohort study. Corresponding virus infection rates (cases per 10,000 person-years) that were reported in the general population in Finland are also shown (yellow lines), although data for rhinoviruses and parainfluenza viruses were not available.(From Vesa S, Kleemola M, Blomqvist S, et Epidemiology of documented viral respiratory infections and acute otitis media in a cohort of children followed from two to twenty-four months of age. Pediatr Infect Dis J 574-581, 2001. Used with permission of Lippincott Williams & Wilkins.)© 2008 Lippincott Williams & Wilkins
Respiratory viruses cause infections in children at specific ages. RSV causes the most severe LRI in children less than 1 year of age, with a peak occurring at a mean age of 3 months.^29^ The peak age for hMPV infection is later than that for RSV,^30^ but the majority of children have had clinical or subclinical infection by 2 years of age. Similarly, hPIV infections occur at an older peak age than RSV. Human parainfluenza virus-1 causes LRIs predominantly in infants aged 7 to 36 months, with peak infection occurring in the second and third years of life. The peak infection of hPIV-2 occurs in the second year of life, but hPIV-3 differs in that it infects infants younger than 6 months and most children experience infection in the first year of life. All age groups are at risk of infection from influenza type A and B viruses, but preschool-age children in day care and school-age children have the highest infection rates.^31^ The very young, the elderly, and those with chronic conditions experience more severe disease. Rhinoviruses and coronaviruses commonly cause URI and infect individuals many times throughout their lives. Rhinoviruses are also clearly associated with LRI in children, but their role remains to be elucidated.^32^
The common respiratory viruses are often associated with specific clinical syndromes, although most can cause infection at any level in the human respiratory tract.
RSV is clearly the most important pathogen associated with bronchiolitis and pneumonia in infancy,^33^ but it can infect all age groups, causing influenza-like illness.^34^
Infection of young children with hMPV, a newly described virus, causes illness that resembles hRSV bronchiolitis but also upper respiratory tract disease and diarrhea with fever.13, 35, 36, 37, 38 Metapneumovirus now surpasses the parainfluenza viruses as the second most common cause of bronchiolitis and pneumonia among young children.
The parainfluenza viruses (hPIV 1-4) are also an important cause of LRIs and regularly cause pneumonia, bronchiolitis, and croup among infants and young children. The most frequent are hPIV-1 and hPIV-2 infections. hPIV-1 is most commonly associated with croup, whereas hPIV-3 is an important cause of bronchiolitis and pneumonia in young infants. hPIV-2 is most commonly associated with croup, but all respiratory syndromes have been described. Studies rarely identify hPIV-4, although this virus has also been associated with all respiratory syndromes.^39^
Influenza viruses can cause any of the typical respiratory syndromes. Very young infants often present with fever only and no specific lower respiratory tract symptoms. School-age children and adolescents most often present with symptoms of classic influenza (i.e., febrile tracheobronchitis with myalgia and cough).^1^
The most common lower respiratory syndrome caused by adenoviruses is pneumonia, but all syndromes can occur.^40^ Damage to bronchial architecture can occur with certain adenovirus strains, leading to life-threatening infections, and to bronchiolitis obliterans and bronchiectasis.
Rhinoviruses are the most ubiquitous respiratory pathogens. They cause the majority of cases of the common cold and may also commonly be associated with LRIs in children and adults.^32^
The coronaviruses (hCoV-229E and hCoV-OC43) are a frequent cause of URIs in children and adults.^41^ Human bocavirus ^42^ and the newer coronaviruses (hCoV-NL63 and hCo-HKU1)16, 19, 43 have been associated with respiratory tract infection in children, but their epidemiology remains to be fully elucidated.
To prove a diagnosis of “pneumonia” according to the first, pathologic definition requires proof that there is infection of the lung parenchyma and proof that there is airspace consolidation. The definition of ARI or clinical pneumonia, on the other hand, relies on clinical signs only and requires no such proof. The chief difficulty in studying cases of pneumonia, especially for epidemiologists, is that of reconciling these two definitions. Why is this important? When we describe the epidemiology of clinical pneumonia we need to
The difficulties inherent in answering these two questions include the following.
The difficulties and limitations of identifying ARI by microbial cause and radiologic consolidation, particularly in developing countries, have forced epidemiologists to develop tools using clinical pattern recognition to estimate the burden of respiratory disease. The World Health Organization (WHO) guideline for the management of ARIs and pneumonia (Fig. 31-3 ) was developed by Shann and others45, 46 in Papua, New Guinea, from 1980 onward. The aim was a simple step-by-step instruction in recognizing and treating respiratory infection, requiring minimal training, and usable by health workers in developing countries. By 1994, 130 developing countries were using the protocol with or without modification.^47^ The guideline has been incorporated into the global Integrated Management of Childhood Illness (IMCI) program,^48^ which had been adopted by 81 developing countries by 2000. For these reasons, the WHO guideline has become an important tool for measuring the incidence and mortality due to ARI.
Figure 31-3 The modified WHO case management guideline for acute respiratory illness.(Used with permission. This chart and its explanation can be found at http://www.who.int/child-adolescent-health/Emergencies/ARI_chart.pdf.)
The strengths of the WHO case management guideline for epidemiological study of ARI are as
A meta-analysis of nine community-based trials49, 50 has shown that application of the WHO case management system reduced pneumonia mortality by 36% and total mortality by 24% in the 0- to 4-year-old age group (Fig. 31-4 ). The impact is most likely due to improved recognition of antibiotic treatable pneumonia due to S. pneumoniae and H. influenzae, but identification of malnutrition and other health factors may also contribute.
Figure 31-4 Risk ratio and 95% confidence interval for reduction in total and pneumonia mortality in children 0 to 4 years old, showing estimates in individual studies and random effects, pooled estimates, and weights for each study.Letters in study column refer to study locations (G, Gadchiroli, India; J, Jumla, Nepal; N; Navangwal, India). See source article for complete study references. (From Sazawal S, Black RE: Effect of pneumonia case management on mortality in neonates, infants, and preschool A meta-analysis of community-based trials. Lancet Infect Dis 547-556, 2003.)
Compared to a radiologic definition of pneumonia, the WHO guideline lacks specificity due
In two studies in The Gambia^61^ and Peru,^62^ radiographs confirmed pneumonia in 24% and 36% of cases, respectively, identified by WHO criteria.
The “verbal autopsy,” commonly used for gathering mortality data in developed countries, consists of a questionnaire (including the WHO criteria for ARI) administered to families about the final illness. This is a simple method of obtain ing circumstantial data regarding causes of death without the cultural problems often associated with medical autopsy. Verbal autopsies have a range of reported sensitivity for pneumonia of 28% to 72% and specificity of 60% to 90%.^49^ Limitations of the verbal autopsy are similar to those listed for the WHO guideline; such as possible inclusion of deaths from malaria (where prevalent) among deaths from ARI^63^ and frequent confounding of cause of death by comorbidities such as malnutrition, measles, or human immunodeficiency virus (HIV) infection in poor countries.^49^ ARI is an associated cause or final mode of death even more often than it is a direct cause of death.^64^
We should bear in mind the sensitivity and specificity of the tool used when comparing data. Compared with children in developed countries, children in developing countries have high exposure to risk factors, high rates of bacterial infection, low access to medical care, and high incidence of and mortality from pneumonia. The WHO criteria for ARI have been deliberately chosen to have high sensitivity, accepting the cost of lower specificity. This may have the effect of exaggerating differences in estimates of ARI between developed and developing countries. On the other hand, many researchers state that their best estimates of ARI in developing countries remain conservative and are likely to underestimate the rates. These differences in method should be kept in mind when discussing the estimates below.
Global estimates and national estimates of ARI incidence and mortality usually depend on data derived indirectly by curve fitting, extrapolation, and applying one proportion to another. Studies at different time points and using different methods may be included. Many of the incidence studies go back to the 1980s,^65^ whereas mortality estimates are based on more recent total and cause-specific mortality data.
The majority of international studies of the epidemiology of ARIs deal with children under the age of 5 years. There are fewer data for older age groups; nonetheless, several studies66, 67 have shown a decreasing incidence with age. For the remainder of this section, we confine our discussion to the child less than 5 years of age.
Rudan and associates^65^ recently estimated worldwide incidence for ARI in children under 5 years of age. They performed a meta-analysis of 28 studies completed mostly in the late 1980s in developing countries. They sought to confirm the directly reported rates in these studies with indirectly calculated rates using case-fatality, rates of severe pneumonia, and case-fatality of severe pneumonia. Their best estimate for the incidence of ARI in the developing world was 0.29 events per child-year, or 150.7 million new cases of ARI per year, of which 11 to 20 million are severe enough to require hospital admission.
Rudan and associates were unable to find studies for the developed world that used active surveillance and WHO criteria for ARI. They based their estimate of 0.026 event per child-year (or 2.1 million cases of pneumonia per year) on four large population-based studies of community-acquired pneumonia in the United States and Europe.
The recent UNICEF/WHO report on pneumonia^68^ indicates that in 2004, 15 countries accounted for three quarters of childhood pneumonia cases worldwide, amounting to approximately 113 million cases. These countries, and the estimated number of cases in 2004, were India (44 million), China (18 million), Nigeria and Pakistan (7 million each), Bangladesh and Indonesia (6 million each), Brazil and Ethiopia (4 million each), Democratic Republic of the Congo and Philippines (3 million each), followed by Afghanistan, Egypt, Mexico, Sudan, and Vietnam (2 million each).
By country, the percentage of annual deaths of children under 5 years that are due to ARI is related in a logarithmic fashion to the total under 5-year mortality (Fig. 31-5 ).^49^ Figure 31-6 shows the estimated percentage range of deaths from lower ARI among children of each country in 2000.^49^ These data were estimated by extrapolating to the year 2000 the 1999 WHO total under-5 mortality data by country, and using the blue fitted curve in Figure 31-5 to estimate the percentage of deaths due to ARI. The authors estimated that between 1.6 and 2.2 million children died of ARI in 2000 and that 40% of these deaths occurred in Africa and 30% occurred in Southeast Asia. Mortality from ARI in excess of 25% of total under-5 deaths was estimated for Afghanistan, Sierra Leone, and Niger. The regions with the lowest proportions of deaths due to ARI were in Western Europe, North America, and Australasia. Other respiratory deaths in young children, estimated for 1998, fell into the following upper respiratory infection and otitis media—37,000, neonatal pneumonia—215,500, pertussis—287,000, measles—594,700, and AIDS-associated ARI—87,500.^69^
Figure 31-5 Percentage of deaths due to acute lower respiratory tract infection, adjusting for bias in verbal autopsies. The blue curve is a weighted log-linear fit to the data points, which are assembled from a number of studies. The red and green curves are (respectively) expected values and their 95% confidence intervals after correcting for the calculated underestimation and variability of reporting deaths from acute lower respiratory tract infection because of the use of verbal autopsies in many of the studies.(From Williams BG, Gouws E, Boschi-Pinto C, et Estimates of world wide distribution of child deaths from acute respiratory infections. Lancet Infect Dis 25-32, 2002.)
Figure 31-6 Estimates of the percentage of childhood deaths that are attributable to acute lower respiratory tract infection by country in 2000. The last category includes values up to 36%.(From Williams BG, Gouws E, Boschi-Pinto C, et Estimates of world wide distribution of child deaths from acute respiratory infections. Lancet Infect Dis 25-32, 2002.)
Table 31-2 gives an updated summary of mortality due to ARI from the WHO 2005 report^70^ by region averaged for the years 2000 to 2003. The numbers have slightly increased but are otherwise similar to those of Williams and associates.^49^ Table 31-3 gives a summary of global statistics for ARI in children under 5 years of age, divided into those for the developing and those for the developed world. These figures are based on Rudan and colleagues^65^ and the recent UNICEF/WHO report.^68^
If for the moment we ignore the potential errors due to the limitations mentioned above, it would seem that 90% of the child deaths from ARI occur in the developing world, with 75% of them in Africa and Southeast Asia. ARIs constitute about 20% of total mortality in the developing world but only 2% of total mortality in the developed areas of Europe, the Western Pacific, and the Americas. The differences in estimation and comparison are unlikely to have caused such large discrepancies.
Table 31-4 shows well-established risk factors for developing ARI or pneumonia. Risk factors such as malnutrition are prevalent in developing countries, whereas vehicle emissions may be higher in developed countries.
Risk factors for dying from ARI (almost all from pneumonia) include most of the same factors, but comorbid diseases (especially diarrhea, malaria, measles, tuberculosis, and HIV infection) further increase the mortality risk.49, 71 Lack of adequate health care is also an important associated factor in deaths from pneumonia. Geography, education, access, and accessibility are all factors in the adequacy of health care. Where there is access to health care, the cost of life-saving treatment for pneumonia is relatively small.
According to a Lancet editorial in 2003,^72^ 26% of the world's children under the age of 2 do not receive basic diphtheria, tetanus, and pertussis vaccination, 58% do not receive exclusive breastfeeding for the first 4 months of life, 25% have malnutrition, and 40% do not receive appropriate antibiotic treatment for pneumonia. These risk factors are of course bound together by many strands within communities. As Bawaskar^73^ has pointed out, illiteracy is a major factor in infant malnutrition, and low income and illiteracy are enmeshed with impoverished, unsanitary and crowded living conditions, high rates of HIV infection, and lack of access to health care.
Black and colleagues^71^ have estimated that as many as 50% of the deaths from ARI/pneumonia in children are attributable to being underweight (Fig. 31-7 ). A study in Costa Rica in the early 1970s documented a 12-fold increased risk (71% versus 6%, respectively) of pneumonia among malnourished compared with normally nourished children.^74^
Figure 31-7 Distribution of global deaths by cause. Bars indicate uncertainty bounds. *Work in progress to establish the cause-specific contribution of being underweight to neonatal deaths.(From Black RE, Morris SS, Bryce J: Where and why are 10 million children dying every year? Lancet 2226-2234, 2003.)
Rice and coworkers^75^ presented a comprehensive review of the recent literature regarding the effects of malnutrition on death from diarrhea, ARI, malaria, and measles. They summarized three community studies and 12 facility-based studies. The results were consistent across all studies showing a major association between death from ARI and low weight-for-age (W/A) Z-scores or median W/A less than 80% of median. The odds ratios, risk ratios, or relative risks of dying from respiratory infection compared with that for normally nourished children ranged very widely from single figures up to an odds ratio of 26 for W/A Z-score = -2.0 (compared with >0) in a Brazilian study and an adjusted relative risk of 27 for children whose W/A was less than 60% of the local reference median (compared to ≥90%) in a Philippines study. All studies that analyzed risks by degree of malnutrition found a dose-response effect.
Figure 31-8 , taken from Victora and colleagues^76^ and based on Kirkwood and colleagues,^77^ shows that 84% of deaths from pneumonia occur in the first 18 months of life and indicates the potential impacts of nutritional problems at different ages. Victora and colleagues' estimated reduction in pneumonia mortality by region and by intervention is shown in Table 31-5 .
Figure 31-8 Relations between nutritional risk factors for pneumonia mortality and distribution of pneumonia deaths by age. RR, relative risk.(Redrawn from Victora CG, Kirkwood BR, Ashworth, A, et Potential interventions for the prevention of childhood pneumonia in developing Improving nutrition. Am J Clin Nutr 309-320, 1999.)© 2008
Graham and coworkers^78^ have summarized recent data for the respiratory effects of HIV infection in African children. Approximately 90% of the HIV-infected children in the world come from sub-Saharan Africa. HIV infection greatly increases the incidence of and mortality from pulmonary disease, mostly in children under 5 years. Conversely respiratory disorders are the most common cause of death in HIV-infected African children. For children hospitalized for severe pneumonia, HIV-infected children in Malawi were three times as likely to die, and in South Africa six times as likely to die, as were non–HIV-infected children.
The high incidence of opportunistic infections (such as *Pneumocystis jirovecii—*formerly known as Pneumocystis carinii) of the respiratory tract in HIV infection is well known. The common pathogens found in HIV-infected children with pneumonia are, in the United States, S. pneumoniae and Salmonella spp., and in Africa, S. pneumoniae, H. influenzae, Staphylococcus aureus, Escherichia coli, Klebsiella spp., and nontyphoidal Salmonella spp.^78^
HIV infection is commonly associated with other risk factors for pneumonia including poverty and malnutrition, such as children with marasmus.^78^ As a result, it can be difficult to determine the relative importance of the HIV infection compared with other risk factors in determining the nature and outcome of pneumonia in regions with high HIV prevalence.
Among causes of ARIs in young children, the two organisms responsible for the largest number of cases (and 70% of the deaths)^57^ are S. pneumoniae and H. influenzae, the latter including nontypable strains, type B (Hib), and sometimes other typable strains.^79^ We now have the means to prevent pneumonia due to Hib and to many strains of S. pneumoniae. Madhi and colleagues^80^ demonstrated that Soweto children (both HIV positive and negative) immunized with pneumococcal conjugate vaccine had 31% fewer episodes of virus-associated pneumonia compared with placebo controls. This seems to imply that virus-associated pneumonia commonly involves mixed infections with bacteria.
Isolation rates of S. pneumoniae in pneumonia are very dependent on sample and identification method and are highly variable in studies in developing and developed countries.79, 81 Berman^82^ reported an overall isolation rate of 27% in 14 studies, and 60% of the studies reported isolation rates greater than 30%. The estimated incidence of pneumonia due to S. pneumoniae also varies greatly. Incidence rates from 18.1 to almost 200 per 100,000 children per year have been described in developing countries.79, 83, 84 Incidence rates estimated in Southern California were 17 per 100,000 per year in 1996.67, 85 Serotypes 1, 2, 5, 7, 9, 14, 15, 18, 19, and 23 are common in both developing and developed countries.^79^ Despite the frequency of isolation of S. pneumoniae, there are few specific estimates of pneumonia mortality due to this organism.
The peak season for pneumococcal pneumonia in developed countries is winter66, 86 (Fig. 31-9 ), closely corresponding to peak isolation rates of RSV and influenza. In tropical countries, studies have reported either little seasonal variation^84^ or small peaks in the hot season and the monsoon season^83^ (Fig. 31-10 ). The peaks observed could be explained either by secondary infection on the heels of seasonal viral infections or (in parallel to virus infections) by increased interpersonal transmission of bacteria during seasons when people spend more time indoors.
Figure 31-9 Weekly isolation of winter respiratory viruses RSV (red line) and influenza (blue line) and frequency of invasive pneumococcal disease (green line) from January 1, 1995, through June 30, 2002.(From Talbot TR, Poehling KA, Hartert TV, et Seasonality of invasive pneumococcal temporal relation to documented influenza and respiratory syncytial viral circulation. Am J Med 285-291, 2005.)© 2008
Figure 31-10 Seasonal distribution of pneumococcal pneumonia in 103 children in Upper River Division, The Gambia, 1989 to 1991.(Data from O'Dempsey TJ, McArdle TF, Lloyd-Evans N, et Pneumococcal disease among children in a rural area of west Africa. Pediatr Infect Dis J 431-437, 1996. Used with permission of Lippincott Williams & Wilkins.)© 2008 Lippincott Williams & Wilkins
Studies in developing countries have indicated that both total child mortality and mortality due to pneumonia could be lowered by the use of pneumococcal vaccines. Black and coworkers,^87^ in a double-blind randomized controlled trial of heptavalent pneumococcal conjugate vaccine in California, reported a 32.2% reduction in pneumonia with positive radiographs in children under 1 year, 23.4% in children under 2 years, and 9.1% in children over 2 years old. Cutts and coworkers^88^ have reported a randomized double-blind placebo controlled trial of nine-valent pneumococcal conju gate vaccine in Gambian infants less than 1 year old. They estimated (based on intention-to-treat analyses) a reduction in first episodes of pneumonia of 6%, reduction of admissions to hospital of 13%, reduction of admissions due to potentially invasive pneumococcal disease of 19%, and reduction in all-cause mortality of 14%. Vaccine efficacy was 77% against invasive pneumococcal disease due to vaccine-related serotypes and 50% against invasive disease due to all serotypes.
Levine and coworkers,^89^ in a 1998 review, estimated that H. influenzae (all strains) caused 16% of all pneumonia deaths not due to measles or pertussis or 482,000 deaths from pneumonia each year in children under 5 years in developing countries. Peltola^90^ conservatively estimated the annual prevaccination incidence of Hib pneumonia in children under 5 years in developing countries at 300 per 100,000 based on Gambian studies in the 1990s.90, 91 This represents 1.7 million cases and 220,00 to 400,000 deaths with a case-fatality rate of 13% to 24%. In developed countries, Peltola estimated a prevaccination incidence of 6 per 100,000, representing 5000 cases, 250 deaths, and a case-fatality rate of 5%.
Nontypable strains have been found to be as commonly or more commonly associated with pneumonia in several studies,79, 90 but only in developing countries. Levine and colleagues^89^ reported that Hib accounted for a mean of 35% of all H. influenzae pneumonia cases in three studies of all H. influenzae pneumonias but for a mean of 71% of bacteremic H. influenzae pneumonias in eight studies. If most deaths occur in bacteremic pneumonia, as is supposed, then Hib may be responsible for up to 70% of the deaths from H. influenzae pneumonia in under-5 children.
Vaccination for H. influenzae type B has dramatically reduced all causes of invasive Hib in the developed world^92^ (Fig. 31-11 ).
Figure 31-11 Invasive Hib infections by age, 1990 to 2001, England and Wales, combined PHLS HRU/CDSC data.(From McVernon J: Hib surveillance and remaining HIB disease in the UK. Paper presented at Global Reduction of Hib Disease Meeting, September 22-25, 2002, Scottsdale, Arizona.)© 2008
Trials of Hib conjugate vaccine in developing countries have included prospective case-control studies in Chile^93^ and Brazil^94^ and a double-blind randomized placebo controlled trial in The Gambia.^95^ Vaccine efficacy in young children ranges from 21% to 31% against radiology-confirmed pneumonia from all causes in these studies. The Gambian study showed reduction of oropharyngeal carriage of Hib.^96^
Levine and co-workers in 1998^89^ estimated the potential impact of routine immunization of infants with 3 doses of Hib conjugate vaccine in developing countries (Table 31-6 ). The estimates include all Hib disease (the majority being pneumonia and meningitis). They suggested that 60% of deaths and 67% of cases would be prevented if no herd immunity were operative. In the presence of herd immunity over 300,000 deaths (83% of deaths) and 2.5 million cases (85% of cases) might be prevented. Miller^97^ estimated that among Asian countries, about 136,000 (87%) Hib deaths could be prevented annually with incorporation of Hib vaccine into the immunization programs at a cost of between 0.1% and 3.0% of per capita gross national product per child younger than 5 years. However, a recent Hib vaccine probe trial among 55,073 children in Lombok, Indonesia,^98^ found no protective effect against radiology-confirmed pneumonia despite a protective effect against clinically defined pneumonia and against meningitis. Various reasons are discussed in the paper and the accompanying editorial.^99^
ARI and pneumonia contribute a massive burden of death and morbidity to young children and much more so in developing countries than in developed countries. Vaccination strategies to prevent disease are well proved for single-agent diseases with obligate human hosts. However, vaccine implementation, acceptance, and coverage are always going to offer challenges when different political, economic, health, and cultural systems have to be engaged. Other major factors that will be required to decrease the morbidity and mortality due to ARI in children must include the reduction or eradication of poverty, malnutrition, and HIV infection and improve ments in air quality through reduction of smoke exposure from cigarettes and biomass fuels.
Infectious diseases are the leading cause of death among young people under the age of 50 years.^100^ The complex matrix of these diseases from endemic diseases, which pose an ongoing threat, through to new emerging and reemerging diseases that present new challenges^101^ is shown in Figure 31-12 . Respiratory pathogens, including SARS, influenza, and tuberculosis, are some of the emerging and reemerging threats that have recently received or are receiving ongoing attention as global public health concerns. These infections, many of which are zoonotic in origin, are creating unprecedented challenges, and spurring the development of new approaches. The application of epidemiology simulation modeling to identify the best public health responses under a variety of conditions^102^ and for specific interventions with pandemic influenza^103^ are examples.
Figure 31-12 Global examples of emerging and reemerging infectious diseases. Red represents newly emerging diseases; blue, reemerging/resurging diseases; black, a “deliberately emerging” disease.(From Morens DM, Folkers SK, Fauci AS: The challenge of emerging and re-emerging infections diseases. Nature 242-249, 2004.)© 2008
Of all the viruses that infect the human respiratory tract, influenza viruses cause the predominant number of serious acute respiratory tract illnesses.^104^ There are three types of influenza virus, A, B, and C, of which the influenza A and B viruses are clinically relevant in humans. Influenza B viruses have a human reservoir, whereas influenza A viruses have a reservoir in aquatic birds and are antigenically diverse. Influenza A viruses have two major antigenic surface proteins, the hemagglutinin (H) and neuraminidase (N). We recognize 16 different H antigens (named H1–16) and nine different N antigens (N1–9), and unique combinations of H and N antigens are called influenza A subtypes. All of these subtypes have been found circulating among wild aquatic birds, their primary natural reservoir. From time to time, these viruses cross the species barrier, with some becoming established in another avian or animal species. In the past century, the influenza A subtypes H1N1, H2N2, and H3N2 have been able to infect and establish sustained transmission among humans, whereas the H5N1 subtype has become established in domestic poultry (Fig. 31-13 ). The constant evolution of influenza A and B viruses occurs through the accumulation of mutational changes in the H and N antigens. This antigenic drift leads to the selection of new variants and regular epidemics of disease, while reassortment of the H and N genes of different influenza A subtypes (antigenic shift) leads to the emergence of a novel virus and pandemic influenza.^105^ However, it is becoming clear that the emergence of human influenza virus lineages can occur through avenues other than drift and shift.^106^
Figure 31-13 Time course of global spread of human influenza virus A subtypes and human outbreaks of avian influenza viruses.(Redrawn from Fauci AS: Emerging and reemerging infections The perpetual challenge. Acad Med 1079-1085, 2005.)© 2008
Epidemics of influenza occur almost every year. They are caused by new variants of influenza A and B viruses that have evolved through antigenic drift, allowing them to evade the host's immune defenses. This requires regular updates of the composition in influenza vaccines. In countries with temperate climates influenza activity peaks during the winter months with influenza A epidemics occurring every 1 to 2 years and influenza B circulating every 2 to 4 years.
Influenza incidence and illness are high in children. Attack rates can be 40% or more in preschool-aged children and 30% in school-age children, which is higher than the 10% to 20% rate commonly observed in young adults.26, 33, 107, 108, 109
Children have an important role in spreading influenza. School provides an ideal environment for the spread of respiratory viruses, and school-age children serve as the main channels through which influenza A and B virus infections are introduced into households.107, 109, 110 Exacerbations of COPD in adults were found to be correlated with epidemics of viral infections including influenza A and B in school children and to decline during school holidays.^111^ In young children, influenza virus is detectable 1 to 3 days after infection and shedding often persists for 10 days to 3 weeks.^112^
During childhood, influenza is the most significant cause of acute respiratory illness leading to hospitalization,^113^ outpatient visits,^108^ and courses of antibiotics in children of all ages.^114^ Healthy children younger than 1 year are hospitalized at rates similar to those for adults at high risk for influenza.^115^ The rate of hospitalization decreases with age. Similarly high hospitalization rates have been reported among children in the subtropics compared with those reported in temperate regions.^116^
Human pandemics of influenza have been reliably described since the 16th century, with an average of three occurring every 100 years.^117^ Over the 20th century, there have been the 1918-1919 “Spanish” H1N1 pandemic, which caused an estimated 20 to 50 million deaths, mainly in previously healthy persons aged 20 to 40 years old, and the 1957 “Asian” H2N2 and 1968 “Hong Kong” H3N2 pandemics, which caused large numbers of cases and a combined mortality estimated to be more than 3 million deaths, mostly in the very young, the elderly, and people with underlying chronic conditions (see Fig. 31-13).
Recovery of lung tissue from victims of the 1918 pandemic has allowed the isolation of viral RNA and the reconstruction of the complete 1918 pandemic virus in the laboratory.118, 119 These experiments support the hypothesis that the 1918 H1N1 virus was of avian origin and adapted to human infection and transmission. In contrast, the influenza viruses that caused the 1957 and 1968 pandemics are human-avian reassortant viruses, and this difference may be relevant to the severity of the 1918 pandemic.^120^
Human infections and outbreaks following interspecies transmission of highly pathogenic avian influenza viruses have rarely been reported before 1997.^121^ Reports are increasing of human infections associated with direct or indirect contact with infected birds. In 1997, the infection of 18 humans, of whom 6 died, with an avian H5N1 virus raised the level of global concern of a possible human influenza pandemic. In 1999, H9N2 avian influenza infected two children in Hong Kong and there were other cases in mainland China. In 2003, H5N1 and H9N2 infections were confirmed in Hong Kong, while in the Netherlands, a large avian influenza outbreak involved an H7N7 virus. Up to 1000 cases among farmers and poultry workers occurred. Since late 2003, outbreaks of avian H5N1 have been reported among poultry in Southeast Asia.^122^ Human infections and deaths were initially reported in Vietnam and Thailand. This virus has become endemic in domestic poultry in Asia and has spread globally after infecting migratory waterfowl.^123^ Subsequently, there have been reports of human infections in an increasing number of countries. Clusters of human infection are small, suggesting that if human-to-human transmission is occurring, it is very inefficient.
As this H5N1 virus continues to circulate in and be spread by domestic and migratory avian species, there is an ongoing risk of human infection and a threat of the emergence of a human pandemic virus. Whether the H5N1 virus or one of the other potential pandemic subtypes (H2, H5, H7, or H9 viruses) will adapt to efficient human-to-human transmission remains unknown. Currently, no mechanism of prediction of the emergence of novel influenza A viruses exists, highlighting the importance of the ongoing global surveillance of animal and human influenza viruses by the WHO.
SARS is an acute viral respiratory syndrome caused by a novel coronavirus, the SARS coronavirus (SARS-CoV), and rec-ognized as a global threat in mid-March 2003. The virus is an animal virus that has crossed the species barrier to humans.124, 125 The first known cases are believed to have occurred in Guangdong Province, China, in November 2002.^126^ Early cases were associated with occupational exposure to infected animals^127^; however, once the number of cases started to increase, health care workers and their close contacts were at greatest risk of infection. The epidemic was characterized by “superspreading events,”128, 129, 130 which seeded outbreaks in Canada, China, Hong Kong, Taiwan, Singapore, and Vietnam (Fig. 31-14 ). By July 2003, the international spread of SARS-CoV presented a global public health threat resulting in 8098 SARS cases in 26 countries and 774 deaths.^131^ The epidemic caused social and economic disruption in areas with sustained transmission of SARS, and on the travel industry internationally, in addition to the impact on health services directly.
Figure 31-14 The global spread of SARS. The number of probable cases of SARS and the date of onset of the first case in each country (or group of countries) is denoted. The countries denoted in red are those where substantial local transmission occurred.(From Peiris JS, Guan Y, Yuen KY: Severe acute respiratory syndrome. Nat Med 10(12 suppl):S88-S97, 2004.)© 2008
Since July 2003, four sporadic community-acquired cases have occurred in Guangzhou, Guangdong Province, China,^132^ while three incidents have been attributed to exposures in laboratories.125, 133
The natural reservoir of SARS-CoV has been identified as the Chinese horseshoe bat.^134^ Although many animals have been investigated as possible reservoirs,124, 135 bats are well suited to transmit zoonotic disease, as many people in Asia eat bats and use their feces for medicines.
SARS runs a more benign and shorter clinical course in young children (less than 12 years of age) during the acute phase.^136^ Infants born to mothers with the disease did not acquire the infection through vertical transmission. No deaths were reported in children.^137^ Children appear to acquire the infection by close-contact household exposure to an infected adult.^138^
Tuberculosis (TB) remains a major global public health problem. It is estimated that in 2004, one third of the world's population was infected with the mycobacterium bacillus that causes TB and almost 4 million were smear positive.^139^ A number of factors have facilitated the resurgence of tuberculosis. These include the advent of the AIDS epidemic, emigration from countries where prevalence of TB is high, noncompliance of patients, transmission in high-risk environments, and the coincident increase in the number of cases of multidrug-resistant tuberculosis (MDR-TB).
The estimated TB incidence globally is shown in Figure 31-15 .^140^ The largest number of cases occurs in the Southeast Asia region (190 cases per 100,000 population), which accounts for 33% of incident cases globally; however, in sub-Saharan Africa, the incidence per capita is nearly twice that of Southeast Asia, given by the WHO at 356 cases per 100,000 population in 2004.^139^
Figure 31-15 Estimated tuberculosis incidence rates, 2003.(From the Global Plan to Stop TB 2006-2015. Geneva, World Health Organization, Actions for Life, 2006.)© 2008 World Health Organization, Actions for Life
The WHO also estimated that 1.69 million deaths (27 deaths per 100,000 population) resulted from TB in 2004.^139^ As with cases of disease, the highest number of estimated deaths is in the Southeast Asia region, but the highest mortality per capita (78 deaths per 100,000 population) is in sub-Sahaian, Africa, where HIV has led to rapid increases in the incidence of TB and increases the likelihood of dying from TB (Fig. 31-16 ).^140^ TB accounts for about 13% of AIDS deaths worldwide.^141^
Figure 31-16 Estimated prevalence of HIV infection in TB cases, 2003.(From the Global Plan to Stop TB 2006-2015. Geneva, World Health Organization, Actions for Life, 2006.)© 2008 World Health Organization, Actions for Life
Drug-resistant TB is on the increase in many parts of the world.^142^ The development of resistance is a result of inconsistent or partial treatment of cases. Of particular concern is multidrug-resistant TB, which is defined as resistance to isoniazid and rifampicin, the two most effective anti-TB drugs. It is estimated that 300,000 new cases of multidrug-resistant TB are developing each year.^139^