Authors: Sung Jun Cho, Ja Ryung Yang, Hee Seung Yang, Hea-Eun Yang
Categories: Original Article, High-energy shock waves, Knee osteoarthritis, Pain, Stroke, Ultrasonography
Source: Annals of Rehabilitation Medicine
Authors: Sung Jun Cho, Ja Ryung Yang, Hee Seung Yang, Hea-Eun Yang
To evaluate the effects of extracorporeal shockwave therapy (ESWT) on pain, function, and ultrasonographic features of chronic stroke patients with knee osteoarthritis (OA).
A total of 18 chronic stroke patients (33 knee joints) with unilateral or bilateral knee OA (Kellgren-Lawrence grade ≥1) were enrolled in this study. The patients were randomly allocated to an experimental group receiving ESWT (n=9) or a control group receiving sham ESWT (n=9). For the ESWT group, patients received 1,000 pulses weekly for 3 weeks, totaling to an energy dose of 0.05 mJ/mm^2^ on the proximal medial tibia of the affected knee. The assessments were performed before the treatment, immediately after the first treatment, and 1 week after the last treatment using the the visual analog scale (VAS) for pain; patient perception of the clinical severity of OA; the Korean version of Modified Barthel Index (ambulation and chair/bed transfer); the Functional Independence Measure scale (FIM; bed/chair/wheelchair transfer, toilet transfer, walking, and stairs); and ultrasonographic features (articular cartilage thickness, Doppler activity, and joint effusion height).
The experimental group showed a significant improvement in VAS score (4.50±1.87 to 2.71±1.38) and patient perception of the clinical severity of OA (1.87±0.83 to 2.75±0.46). The bed/chair/wheelchair transfer components of the FIM score also improved significantly (4.12±1.55 to 4.62±1.30). In terms of the ultrasonographic features, increased Doppler activity was observed in the medial knee in the experimental group immediately following ESWT.
It is suggested that ESWT may reduce pain and improve function in chronic stroke patients with OA, and may increase vascular activity at the target site.
Knee osteoarthritis (OA) is a common musculoskeletal disease and is a leading cause of pain and disability worldwide [1]. Knee OA negatively affects physical function and quality of life. The prevalence of radiographic knee OA and symptomatic knee OA in Korean elderly community residents (age >50 years) is 37.3% and 24.2%, respectively [2].
In stroke patients, OA can be a comorbid pathology that has a significant impact on the stroke outcome, particularly considering its increasing prevalence with age [3]. Knee OA can be accelerated due to an alteration of normal biomechanics in stroke patients. Knee OA has a negative effect on ambulation levels in post-stroke hemiplegic patients, and it must therefore be taken into consideration during the planning of a stroke rehabilitation program [3].
Optimal management of patients with knee OA requires a combination of non-pharmacological and pharmacological modalities, and surgical treatment is recommended in intractable cases [4]. Non-pharmacological modalities include education, muscle strengthening, thermal modalities, and transcutaneous electrical nerve stimulation, among others, while pharmacological treatment modalities range from oral medication to intraarticular injections.
In recent decades, extracorporeal shockwave therapy (ESWT) has been widely used for pain relief and treatment of musculoskeletal disorders [5]. The effects of ESWT in patients with plantar fasciitis, calcifying shoulder tendinitis, and lateral epicondylitis of the elbow have been reported, and clinical applications of ESWT in patients with these diseases are widely used [5].
Veterinarians have started using ESWT to treat equine knee OA [67]. It has been reported that the application of ESWT on arthritic joints in animals improves limb motor dysfunction [89]. Recent studies reported that, histopathologically, ESWT has a chondroprotective effect in small animals, such as rats and rabbits [101112]. Since ESWT is non-invasive, has a low complication rate, does not require hospitalization, and is relatively inexpensive compared to other conservative and surgical approaches, it is the preferred treatment option for knee OA. To our knowledge, only a few studies have investigated its use in human knee OA, and these studies have assessed only the clinical outcomes and not the morphological or structural changes in the knee joint.
This study was a pilot study that investigated the effects of ESWT in chronic stroke patients with knee OA in terms of pain, function, and morphological changes assessed with ultrasonography.
The present study was a prospective, double-blind, randomized controlled trial. Patients were recruited from an inpatient rehabilitation center. The inclusion criteria were as chronic stroke of ≥2 years duration; unilateral or bilateral knee OA (Kellgren-Lawrence grade ≥1); intact cognition (Mini-Mental State Examination score>20); and ambulatory ability. Patients with other musculoskeletal conditions that can cause lower extremity pain, secondary causes of arthritis, or history of intraarticular knee injection within the previous 6 months were excluded.
A total of 18 chronic stroke patients with knee OA were recruited, and a total of 33 knees were analyzed in the present study. The study protocol was approved by the Institutional Review Board.
For this randomized controlled study, a randomization sequence was generated by a computer program prior to the start of the study. This procedure was performed by an independent researcher who was not involved in the intervention or assessments. The patients were allocated to 1 of the the experimental group (n=9, 16 knee joints) receiving ESWT or the control group (n=9, 17 knee joints) receiving sham ESWT. Patients were not informed of the group assignment. The patients were then referred to other 2 researchers assessing pain, functional scales and ultrasonographic features of subjects, respectively.
ESWT and sham ESWT were administered by a physiatrist (SJ Cho) majoring in rehabilitation medicine who have performed ESWT treatments for more than 3 years and was not involved in the randomization or assessments. Shockwaves were delivered using Dornier Aries (Dornier MedTech, Wessling, Germany); patients underwent 3 sessions at weekly intervals. For the session, patients were in the supine position with the affected knee flexed and the foot on the table. At each treatment session, the experimental group received 1,000 impulses of shockwave at 0.05 mJ/mm^2^ on the proximal medial tibia of the affected knee. The control group received 1,000 impulses of sham stimulation at 0 mJ/mm^2^ on the same area.
Overall, 3 domains were assessed; pain, functional state, and ultrasonographic features. Evaluations were performed at baseline and 1 week after the final treatment session for all participants, but the Doppler activity on the ultrasonography was performed immediately after the first treatment session. All outcome measurements were assessed by 2 researchers who were not involved in the randomization or intervention.
For the pain evaluation, the visual analog scale (VAS) score [13] and patient perception of the clinical severity of OA (PPOA) [14] were assessed. PPOA is a 5-point scoring system that reflects patient consideration about the effect of knee OA on their daily lives, with 1 indicating very poor and 5 indicating very good.
To assess the functional state, specific domains involving the lower extremity in the Korean version of the Modified Barthel Index (K-MBI) [15] and Functional Independence Measure (FIM) scale [16] were used. Ambulation and chair/bed transfer ability from the K-MBI and bed/chair/wheelchair transfer, toilet transfer, walking, and stairs ability from the FIM scale were extracted.
For the ultrasonographic features, we extracted meaningful domains from the standardized musculoskeletal ultrasound (MUS) scoring system for knee OA [17]. Articular cartilage thickness was measured at the medial trochlea, trochlear notch, and lateral trochlea. Joint effusion height was measured at the suprapatellar recess. Doppler activity was assessed at the medial joint space, medial parapatellar recess, lateral joint space, and lateral parapatellar recess. Articular cartilage thickness was measured in every participant, and the Doppler activity and joint effusion were assessed in 5 participants (8 knee joints) from the experimental group and 4 participants (7 knee joints) from the control group.
All analyses were performed using Statistical Package for Social Sciences (SPSS) software ver. 18.0 (SPSS Inc., Chicago, IL, USA). A paired t-test was used to compare within-group changes in the measured parameters, and an independent t-test was conducted to determine statistical differences in the variables between the 2 groups. The statistical differences of each segment in terms of the Doppler activity data were analyzed using repeated measures ANOVA. Values are presented as mean±standard deviation, and p<0.05 was considered statistically significant.
All 18 patients completed the treatment sessions without any complications. There were no significant differences between the experimental and control groups in terms of the baseline characteristics and parameters (Table 1).
The experimental group showed reduced pain, as measured on the VAS and PPOA scales after the intervention (Table 2). The VAS score was 4.5±1.9 at baseline and 2.7±1.4 after the treatment (p=0.00). The PPOA score was 1.9±0.8 at baseline and 2.8±0.5 after the treatment (p=0.02). The control group showed no significant change in the pain domain. In the functional domain, the control group showed no significant change after all the treatment sessions. The experimental group showed an improvement in the bed/chair/wheelchair transfer component of the FIM score, but not in other components of the FIM or K-MBI (Table 3). The bed/chair/wheelchair transfer score on the FIM scale was 4.1±1.6 at baseline and 4.6±1.3 after the intervention in the experimental group (p=0.03).
The control group showed no significant change after the intervention in any of the domains of ultrasonographic features. The Doppler activity was evaluated using the following scoring system (0, none; 1, less than half of the area of interest; 2, more than half of the area of interest) [17]. An increase in Doppler activity was observed in the medial joint in the experimental group immediately after the first ESWT session. The Doppler activity score of the medial joint was 0.0±0.0 at baseline and 0.8±0.7 immediately after the ESWT (p=0.04). However, a decreased tendency was observed in the Doppler activity at 1 week after all the treatment sessions (0.5±0.5), compared to that obtained immediately after the ESWT (0.8±0.7), although it was not statistically significant (p=0.08). No change was noted in other ultrasonographic findings, such as articular cartilage thickness, joint effusion, and Doppler activity in other parts excluding the medial knee joint (Tables 4, 5).
Increased Doppler activity was observed in diverse parts of the medial knee joint in each patient and most commonly on the distal femoral and proximal tibial surfaces (Fig. 1). In some patients, Doppler activity was also increased on the medial ligament or medial meniscus (Fig. 2).
The present pilot study explored the effects of ESWT in chronic stroke patients with knee OA in terms of pain, function, and ultrasonographic features. Participant recruitment and treatment adherence were considered successful, and there were no adverse events related to the intervention. It is proposed that ESWT may increase site-specific Doppler activity, reduce pain and improve motor function.
ESWT has been applied in various musculoskeletal disorders, including calcifying shoulder tendonitis, lateral epicondylitis of the elbow, and proximal plantar fasciitis. Recently, ESWT has been proposed as a therapeutic modality for knee OA. The exact mechanism underlying ESWT is unknown, but several studies suggest that shockwaves stimulate the early expression of angiogenesis-related growth factors, and then induce the ingrowth of neovascularization that improves blood supply and increases cell proliferation and eventual tissue regeneration to repair tendon or bone tissues [518].
The chondroprotective effect of ESWT in OA has been established in animal studies. Wang et al. [101119] performed a histomorphological examination and immunochemical analysis after the application of ESWT in the osteoarthritic knee in rats. Their studies demonstrated that ESWT protects articular cartilage degradation and improves subchondral bone remodeling in rats. The changes in the Mankin score, Safranin O staining, matrix metalloproteinase 13 (MMP-13), and collagen II were significant indicators of the influence of EWST on articular cartilage. The effects of ESWT in subchondral bone remodeling were supported by the increased vascularization manifested by the changes in the von Willebrand factor (vWF), endothelial nitric oxide synthase (eNOS), vessel endothelial growth factor (VEGF), and by the osteogenesis manifested by the changes in bone morphogenetic protein 2 (BMP-2), alkaline phosphatase, and osteocalcin. Zhao et al. [12] demonstrated that ESWT significantly reduces the nitric oxide level in the synovial cavity of knee joints and chondrocyte apoptosis in rabbits with OA, thereby reducing the progression of OA.
In spite of this, its effects on human knee OA have not been studied sufficiently. There are only 2 previous human studies, and these studies proved that ESWT is effective in reducing pain and improving knee function. However, only the clinical outcomes related to knee OA were assessed, and the substantial morphological or hemodynamic changes in the knee joints were not included [2021].
Prior to the application of ESWT, the target area and dosage had to be determined. There is no stated target site for ESWT in knee OA. In humans, ESWT was applied on a trigger point around the knee or on the patellofemoral and tibiofemoral borders of the knee [20]. In rabbits, ESWT was applied around the knee [12]. In rats, ESWT was applied on the distal femur or proximal tibia, and a chondroprotective effect was seen at either site [11]. As different target sites were chosen in each study, we could not have solely referred to a single study in selecting a target site. Therefore, we have targeted at the subchondral bone of the medial proximal tibia that were commonly conducted in the studies. And we performed ESWT treatments on the same area for all patients for the unity of the intervention method. Also, there were some patients who had no tender point around the knee. For that reason, the tender point was not chosen as a target site. Additionally, in this study, veins, nerves, and ligaments were checked using ultrasound, and ESWT was administered at the target site, avoiding those structures.
Safety was the first priority when determining the appropriate dose. A single bout of ESWT (150 shockwaves of 0.5 mJ/mm^2^) caused degenerative changes in the hyaline cartilage of an adult rat, on molecular and ultrastructural levels [22]. A study on the effects of ESWT on human chondrocytes in vitro revealed a dose-dependent increase in cytotoxicity [23]. Cytotoxicity was below 10% when using energy densities of 0.02 mJ/mm^2^ and 0.06 mJ/mm^2^, but the cytotoxicity increased up to approximately 20% when using 0.17 mJ/mm^2^. A recently published study reported that medium energy (0.093 mJ/mm^2^) ESWT was more effective in relieving pain and improving functional outcomes than low energy (0.040 mJ/mm^2^) ESWT. However, histological or molecular safety evaluations were not carried out in that study [21].
According to a study conducted on human chondrocytes in vitro [23], cytotoxicity was measured below 10% with the dose of less than 0.06 mJ/mm^2^. From the study, we have concluded that a dose of less than 0.06 mJ/mm^2^ was an appropriate level for safety. In addition, a human study concerning the effect of different levels of energy revealed that ESWT energy above 0.04 mJ/mm^2^ was more effective [21]. In consideration of these 2 studies, we applied an ESWT dose of 0.05 mJ/mm^2^ in our study. Also, the latter human study [21] indicated that 1,000 shockwaves of ESWT had no side effects or complications, and so we applied the same times of shockwaves in our study.
In our study, an energy density of 0.05 mJ/mm^2^ was selected based on a previous study of human chondrocytes, even though it was an in vitro study.
In chronic stoke patients, it is difficult to expect functional recovery, especially if a specific comorbidity, such as knee OA, exists [3]. In this study, the bed/chair/wheelchair transfer component of the FIM scale was improved in the experimental group. This included all aspects of transferring from a bed to a chair and back, from a bed to a wheelchair and back, and coming to a standing position, if walking was the typical mode of locomotion for the patient. The chair/bed transfer component of the K-MBI also assessed similar activities, but did not show a significant change after the intervention. This may be because the FIM scale has a more subdivided 7-graded consecutive scoring system than K-MBI, which has 5-graded stepwise scoring system.
The MUS scoring system examines 14 anatomical sites and assesses morphological change, inflammation, and effusion, consequently scoring 61 items. This system displayed substantial reliability and reproducibility [17]. Since assessing 61 items is time consuming and unnecessary, we selected 8 relevant items that had a probability of change. While the other items showed no change after treatment, Doppler activity was increased in the medial joint where ESWT was applied. The increase in Doppler activity was site-specific, but was observed in various structures.
To date, several studies have reported angiogenesis associated with ESWT application for knee OA in animal models, but not in human studies. Therefore, this study is meaningful in that it shows hemodynamic changes in the knee joint in humans as a result of ESWT. This increased vascularization after ESWT might indirectly suggest tissue regeneration or a chondroprotective effect.
Increased Doppler activity was not sustained at 1 week after treatment, but reduction of pain and improvement of function were confirmed in the same period of time. In our study, it was not possible to determine if there was any histological change after the decrease in Doppler activity.
Our study has several limitations. First, this study had a small sample size; however, we will conduct a future study with a larger groups of subjects based on the results of this pilot study. Second, we evaluated only the shortterm outcomes. Wang et al. [11] reported that ESWT showed a time-dependent chondroprotective effect on knee OA in rats, with the most beneficial effect at 4 weeks after treatment. Thus, investigation of the long-term effects on cartilage is needed in future studies. Additionally, we applied only 1 treatment protocol in the experimental group. There has been only 1 study on the ESWT protocol for human knee OA [21]. As such, to establish the optimal regimen with regard to dose, frequency, and location, further investigation is required.
In conclusion, the present study suggests that ESWT may reduce pain and improve function in chronic stroke patients with knee OA and may increase site-specific Doppler activity following ESWT, even though the increase is not sustained and seems to decrease with time. For these reasons, we conclude that ESWT could be one of the treatment methods appropriate for human knee OA. Although the results of this pilot study are very encouraging, further study focusing on the treatment regimen and morphological or hemodynamic cartilaginous changes with a larger sample size will be necessary.