The fetal respiratory system as target for antenatal therapy

Authors: J. Toelen, M. Carlon, F. Claus, R. Gijsbers, I. Sandaite, K. Dierickx, R. Devlieger, K. Devriendt, A. Debeer, M. Proesmans, Z. Debyser, A.J. Deprest

Abstract

The widespread use of prenatal ultrasound has made the fetus a patient. A number of conditions diagnosed as such may require therapy prior to birth. Herein we describe past, current and potential future procedures designed to treat pulmonary conditions in the antenatal period. When congenital cystic adenomatoid malformation (CCAM) is associated with fetal hydrops, treatment is required. Prior to viability this may be in utero resection of the pathologic lung lobe or shunting of cystic lesions. More recently, fetuses with isolated congenital diaphragmatic hernia (CDH) with lethal lung hypoplasia have been offered percutaneous fetal tracheal occlusion to provoke lung growth. A very rare condition is laryngeal atresia, which requires peripartum re-establishment of the airways. As we get more experience with access to the fetal airways, this may open the doors for novel therapies. One of these is gene delivery to treat fetuses with serious monogenic disorders or to induce transient overexpression of certain proteins. We review the individual hurdles that are being met by researchers when designing fetal gene therapeutic strategies, in particular for the fetal lung. Also the use of stem cells for pulmonary disorders is currently explored.

Introduction

The introduction of high resolution ultrasound and wide offering of screening programmes prompted the advent of fetal medicine, next to ‘maternal’ care. In other words, information gathered as a consequence of prenatal imaging and subsequent other diagnostic procedures make the unborn fetus a true patient (Fig. 1). When fetal malformations, genetic diseases or in utero acquired conditions are suspected, management can usually wait until after birth. However, a number of conditions may benefit from antenatal interventions, whether they are non-invasive (such as transplacental pharmacological therapy of fetal infections or cardiac arrhythmia) or invasive (such as fetal transfusion of blood derivates). In that case, the intervention is done in the prenatal period because it is either life saving or may prevent organ damage, with the assumption that potential fetal benefits outweigh the risks of the prenatal intervention (Deprest et al., 2006). The fetal respiratory system is only one target for candidate prenatal interventions. Herein we will describe past, current and potential future procedures that focus on treating pulmonary conditions in the antenatal period, such as congenital cystic adenomatoid malformation and congenital diaphragmatic hernia. Next to reviewing the first two indications, the paper focuses on an indication to come, i.e. fetal pulmonary gene therapy. Since fetal medicine specialists are typically not (yet) familiar with gene therapy, its concept will be extensively introduced before focussing on the fetal lung.

Congenital Cystic Adenomatoid Malformation of the Lung

CCAM is a space occupying lesion that, if large enough, causes mediastinal shift and subsequent fetal hydrops as well as pulmonary hypoplasia. In up to 25% of cases these lesions may be hybrid, i.e.; with a component of bronchopulmonary sequestration. When following such patients, one should take into account that the maximal growth of CCAM is around 28 weeks of gestation. Non-hydropic fetuses have an excellent prognosis with in utero transfer, planned delivery, neonatal resuscitation and evaluation. Survival rate is close to 100% for cystic lesions (Cavoretto et al., 2008). Therefore patients should not be needlessly upset (Aite et al., 2009). Actually, about one in five CCAM-lesions decrease or disappear during pregnancy, maybe by decompression into the bronchial tree or by outgrowth. Postnatal imaging is required as they can still cause infection, pneumothorax or even malignant degeneration at a later age. The place of postnatal resection remains a matter of debate (Wilson et al., 2006, 2008).

At present, not much clinical data are available on the potential of predicting lethal pulmonary hypoplasia by different prenatal imaging techniques in case of CCAM. It is therefore difficult to formulate guidelines when to intervene in utero in order to avoid neonatal respiratory insufficiency. The other important aspect is the occurrence of fetal hydrops, which is a poor prognostic factor. This might be caused by impairment of venous return. Mediastinal shift may also contribute to polyhydramnios. Fetal hydrops typically presents as fetal ascites, pleuro-pericardial effusions and subcutaneous edema and is a poor prognostic sign. A large experience gathered by Adzick et al. demonstrated that in case of fetal hydrops, antenatal intervention is needed to prevent in utero fetal death (IUFD) (Adzick, 2003). Moreover, at that time the mother is at risk for the so-called mirror or Balentyne syndrome, particularly in the presence of placentomegaly. It has been proposed to follow up early diagnosed lesions in terms of developing hydrops and IUFD, by using the ratio of the mass of the lesion over the head circumference (CCAM Volume Ratio-CVR) (Crombleholme et al., 2002). When CVR exceeds 1.6, there would be an 80% risk for fetal hydrops.

In case of hydrops, intervention is warranted. As a first treatment step, maternal steroid administration has been described. Its efficacy and wider place in management still remains to be demonstrated, but due to its minimal side effects and their proven benefit in case of prematurity, they seem to be warranted as an initial treatment (Peranteau et al., 2007; Tsao et al., 2003). When hydrops persists, and presents beyond 32 weeks, delivery should take place in appropriate conditions. Earlier than that, fetal intervention can be life saving as reported by several centers now. Roughly spoken the modality is dependent on the morphologic appearance of the thoracic mass. For microcystic masses fetal lobectomy can be carried out by open fetal surgery, with high survival rates in case hydrops resolves. In a series of 22 cases operated between 21-31 weeks, there were 11 long term survivors, who were developmentally normal (up to 12 years of age) (Adzick, 2003). Hydrops resolved in one to two weeks followed by normalization of the position of the mediastinum and the remaining lung underwent impressive catch up growth. Causes of fetal death despite fetal surgery were termination of pregnancy for Ballentyne syndrome (n = 1), preterm labour and/or chorioamnionitis (n = 2), and fetal hemodynamic compromise leading to intra operative death in 6 fetuses and postoperative death in another 2 cases. To prevent intra-operative deaths all means for resuscitation, such as gaining access to the fetal circulation and appropriate fetal monitoring techniques, are now part of the open procedure. It is obvious that the latter procedure can only be offered at centers familiar with open fetal surgery for this and other conditions. When presenting late in pregnancy, fetal lobectomy can be done on placental circulation (Liechty, 2010).

Macrocystic masses can be reduced. Occasionally percutaneous puncture may be successful. However a more permanent drainage by thoraco-amniotic shunting is a minimally invasive procedure that can be successful even if cysts are multilocular, probably because they are all interconnected (Fig. 2, Fig. 3). The largest experience with shunting was published by the Philadelphia group. Shunts reduce the CCAM-volume by 70%, reverse hydrops, and result in a survival rate of 74% (n = 23; (Wilson et al., 2004)). This success rate has since been confirmed by others (Knox et al., 2006). Thoracic shunting has a reported preterm premature rupture of the outer membranes (PPROM) rate of 15% (Picone et al., 2004). Another yet experimental approach is sclerotherapy (Bermudez et al., 2008). Laser coagulation has been used successfully for bronchopulmonary sequestration, where the feeding vessel can be coagulated under ultrasound guidance (Oepkes et al., 2007). Unpublished data from Leiden & Toronto using this modality in CCAM are also encouraging (Oepkes, personal communication). One must realize that this causes collateral thermal damage to surrounding structures, and thoracic deformation has been described already. It can theoretically also worsen fetal hydrops by swelling of the necrotic mass, thus leading to IUFD.

Isolated Congenital Diaphragmatic Hernia

CDH rarely poses an intra-uterine threat to the fetus, except in case of polyhydramnios (leading to preterm labor) or the one percent of cases succumbing to (unexplained) IUFD. The problem of this condition only arises in the neonatal period, when the baby will struggle with the consequences of pulmonary hypoplasia, leading to respiratory deficiency as well as pulmonary hypertension. In essence CDH babies have smaller and less compliant lungs with fewer airway branches and abnormal pulmonary vessels. When isolated, the condition may be lethal in 30-40% of prenatally diagnosed cases in most Western countries (Colvin et al., 2005; Deprest et al., 2006; Deprest et al., 2005; Gallot et al., 2006; Stege et al., 2003). It has been shown that it is possible to predict outcome for left-sided CDH, which constitutes the vast majority (85%). This is done in mid-gestation by documenting the presence of herniation of the liver into the thorax and a low lung-to-head ratio (LHR) (Jani et al., 2006; Metkus et al., 1996) (Fig. 4). For 0.6  LHR < 0.8 survival is 62% and 78% for 0.8  LHR < 1.0 versus 0% resp. 16% in controls treated by standard postnatal care. Correction for gestational age can be done by expressing the observed LHR as a function of what is expected in gestational age matched normal control (O/E LHR) (Jani et al., 2006; Jani et al., 2007). An O/E LHR < 25% identifies a subgroup that is almost certainly deemed to die despite optimal postnatal care (Fig. 5). None of the currently available postnatal therapies can address their underlying problem of severe pulmonary hypoplasia. As a consequence, prenatal therapy aims at stimulating lung growth prior to birth. This is no longer attempted by anatomical repair, but rather by occluding the fetal trachea. This prevents egress of lung liquid, leading to increased pulmonary stretch and accelerated growth of airways and pulmonary vessels. The timing and duration of occlusion are determining the quantitative and qualitative response of airways and pulmonary vessels (Deprest et al., 2006). In experimental lambs cyclical tracheal occlusion yields growth with an optimal balance between type II and I alveolar epithelial cells. Practically the best clinical proxy to this strategy is temporary occlusion (plug-unplug sequence) (Flageole et al., 1998). Tracheal occlusion is achieved by insertion of an endoluminal balloon, which meets both the needs of tracheal growth as well as it allows its reversion either by percutaneous puncture, fetoscopic retrieval or postnatal reversal (Fig. 6). However, prenatal restoration of the airways makes normal vaginal delivery possible at the local referral center and has a beneficial effect on late neonatal survival (Jani et al., 2005). Extrapolating from animal experiments, insertion of the balloon was planned for 26-28 weeks (pseudoglandular phase) and in utero restoration of airways is realized at 34 weeks (saccular to alveolar phase). The procedure is done percutaneously through a 3.3 mm cannula under local or loco-regional anesthesia and fetal immobilization and pain relief.

The European Fetoscopic Endoluminal Tracheal Occlusion (FETO) Task force recently reported its clinical experience in 210 consecutive cases (Jani et al., 2009). Overall, 48% of infants were discharged from the hospital alive. On the basis of stratified data from the antenatal CDH registry, FETO therefore increased survival in severe cases with left-sided CDH from 24.1% to 49.1%, and in right-sided from 0% to 35.3% (p < 0.001). The backside of fetal surgery is that PPROM within three weeks occurred in 16.7% cases. In the entire cohort, gestational age at birth was 35.3 weeks (median), with just over 30% of patients delivering prior to 34 weeks. This is far less than in the earlier experience in the NIH trial by Harrison et al. (Harrison et al., 2003) (Table 1). Nevertheless, it remains a problem particularly if PPROM or delivery takes place prior to 32 weeks. Survival rate after FETO increases from 20-25% prior to 32 weeks (what is expected when expectantly managed), to 60% thereafter. Another predictor of survival is the observed/expected LHR prior to the procedure (Jani et al., 2006). Pulmonary response is also less when FETO is done beyond 30 weeks (Cannie et al., 2009). Therefore late FETO is only practiced within a trial involving more moderate forms of hypoplasia (Deprest et al., 2009). Postnatal management of this multicentre trial, as well as the one in severe cases, to be kicking off soon, is standardized by a consensus protocol (www.totaltrial.eu) (Deprest et al., 2009).

Airway management on placental circulation – perinatal surgery

In the presence of a potential or actual obstruction of the fetal airways, delivery has to be planned such that functionality can be guaranteed. The EXIT (ex utero intrapartum treatment) procedure was initially most frequently used as a delivery technique for safely establishing upper airways following surgical tracheal occlusion. EXIT is however applicable to any fetal condition (potentially) obstructing the upper airways (Mychaliska et al., 1997). The technical details of this treatment modality have been accurately reviewed by Liechty in a recent monography on fetal surgery (Liechty, 2010). Basically EXIT is an operation on placental support (OOPS), as placental circulation allows the surgeon to work safely on the fetal airways, and, more recently, also to enable sufficient time to create vascular access to set up extra-corporeal membrane oxygenation, or to operate on the fetus for other reasons. Using inhalational anesthesia for maximal uterine relaxation, uteroplacental blood flow and gas exchange are maintained, and amnioinfusion and partial delivery of the fetus keep uterine volume normal.

The list of indications for using this technique has grown over the years but essentially includes airway obstruction due to laryngeal atresia, large tumors, or iatrogenic tracheal occlusion. Intrinsic causes of Congenital High Airway Obstruction Syndrome (CHAOS) are stenosis or agenesis of the larynx, cricoid and/or trachea. Associated malformations such as Fraser or DiGeorge syndrome need to be ruled out. Obviously CHAOS is lethal at birth, when not diagnosed prenatally. This should not be a problem as ultrasound reveals signs such as a dilated trachea and main bronchi, bilateral hyperechogenic and enlarged lungs and a flattened to everted diaphragm (Fig. 1). Ascites or hydrops may complicate the condition in utero and even lead to intrauterine fetal death. Prenatal diagnosis enables the organization of this life-saving operation (Oepkes et al., 2003). The level and extent of obstruction can be determined prior to birth and may be of use when securing airways after birth. Essentially this involves establishing a tracheostomy while the neonate is still on placental support. The head and neck of the fetus are exteriorized, and upper airways are explored with a laryngoscope and bronchoscope. If not patent, a tracheostomy is performed.

In utero gene therapy of the lung

Principle of gene therapy

A number of lung disorders are theoretically eligible for gene therapy (Ennist, 1999). Among these, Cystic Fibrosis (CF) has received most interest. CF is the most common autosomal recessive monogenetic disorder in Caucasians and can be readily diagnosed in the prenatal period. CF patients have two out of a series of well identified mutations in the CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)-gene. In the lung, deficient CFTR results in thick mucus, inflammation and secondary bacterial infection, leading to serious morbidity and eventually death early in life. Other candidate pulmonary disorders such as alpha-1 antitrypsin deficiency, surfactant deficiencies (SP-B protein) and primary pulmonary hypertension may benefit from permanent correction, while conditions such as respiratory distress syndrome or perinatal pulmonary infection may benefit from transient gene expression. Today, treatment of those disorders is often symptomatic but not curative.

Although gene therapy is based on a simple concept, there are multiple obstacles (some confined to the lung), preventing successful clinical gene therapy, which will be discussed here. Some of these can be addressed by offering gene therapy in the prenatal period, other issues relate only to the lung and yet other problems are more generic to gene therapy itself. For simplicity we will discuss the concept of gene therapy considering CF as a prototypic disease, but many analogies to other conditions can be made.

Gene transfer agents (GTA) for Cystic Fibrosis

Soon after the cloning of the CFTR gene in 1989, the concept of gene therapy was raised and the first clinical trials started in 1993 (Griesenbach et al., 2009). Clinical trials with non-viral vector systems, i.e. naked DNA, DNA-protein complexes and liposomes showed only transient and partial correction of transepithelial chloride transport, proving the need to optimize the transfer efficiency, improve transgene expression and investigate repeat administrations (Hyde et al., 2000).

The first viral vector system to be investigated for CF clinical trials was the adenoviral vector (AdV). Also here, transduction efficiency in CF patients was relatively low (Grubb et al., 1994; Joseph et al., 2001). The host developed a specific immune response with substantial inflammation to the vector, with as a consequence loss of transgene expression levels below detection limits already after 2 to 4 weeks (Rosenecker et al., 1996). At present the use of AdV is restricted to preclinical research. In 1999, a first clinical trial with a recombinant adeno-associated viral vector (rAAV) was initiated. The virus from which it originates is a non-pathogenic, replication defective virus with a lesser immunogenic potential than AdV. rAAV are capable of transducing non-dividing cells and achieving medium to long term gene expression. Their packaging capacity however is relatively small, making the incorporation of full-length cDNA of CFTR (4.4 kb) with an accompanying promoter challenging. Up till now, all clinical trials for CF have been conducted with vectors containing the capsid of AAV serotype 2 (rAAV2/2). However, gene transfer efficiency to the airways and reversal of biological activity was limited (Carter 2005; Moss et al., 2007). Therefore clinical trials with rAAV2/2-CFTR have been aborted. Recent data from animal models suggest that several of the newly discovered and engineered serotypes have a better tropism for the airway epithelium which might improve results and could overcome possible problems with repeat administrations (Limberis et al., 2009; Limberis et al., 2006). Also in a fetal/neonatal setting, several AAV serotypes have been used to target the fetal lung of rodents (Fleurence et al., 2005; Garrett et al., 2003) and rabbits (Boyle et al., 2001). rAAV2/6.2, a novel serotype that was shown to be more efficient for airway epithelium transduction in adult mice than other AAV serotypes (Limberis et al., 2009), has recently been tested in fetal mice by direct intratracheal injection and robust expression levels as well as stable gene transfer were evidenced up to 1 postnatal month (Fig. 7) (Carlon et al., 2010).

Retroviruses are able to integrate the transgene into the genome of the targeted cells, providing long-term expression. Lentiviral vectors (LV) are a subdivision of retroviruses with unique characteristics. Human Immunedeficiency Virus (HIV) is the best known and studied virus of this family. HIV-derived vectors are able to transduce slowly and even non-dividing, or terminally differentiated cells, thus theoretically they can incorporate a transgene into the genome of stem cells. LV can also easily be pseudotyped, i.e. adjust their envelope so that their tropism for certain target populations increases. For instance, the affinity for respiratory cells can be adjusted by equipping the vector with an Ebola envelope (Kobinger et al., 2001). LV seem the best candidate vector system for stable transduction of respiratory stem cells, hence correction of CF. An integrating vector system however has the theoretical risk of insertional mutagenesis, i.e. the inadvertent change of normal gene regulation. This was observed in the Severe Combined Immunodeficiency Syndrome (SCID)-trial, where children treated with retroviral vectors developed leukemia (Cavazzana-Calvo et al., 2005). Gene transfer into the germline is another theoretical problem, but none of these complications have so far been described for HIV-based vectors.

The initial clinical CF-gene therapy trials achieved only a modest level of functional CFTR reconstitution in vivo and unveiled the physical and physiological barriers, which protect the airways and restrict exogenous gene delivery. The following paragraph describes these pulmonary defense mechanisms more into detail and where relevant, particular issues or solutions relating to fetal pulmonary gene therapy.

Advantages and risks of fetal gene therapy

When revising the experimental data of postnatal gene therapies, several problems become apparent. These can be summarized as (1) sufficient transgene expression requires prohibitively large amounts of GTA; (2) the underlying defects usually have already caused extensive or even irreversible damage; (3) most adult tissues proliferate less than fetal cells, hence are typically less optimally transduced by integrating vectors; (4) host immune responses, either pre-existing or secondary to vector delivery, may rapidly eliminate transgene expression and prevent future interventions. Early gene transfer, in the neonatal or even fetal period, may overcome some, if not all of these obstacles. Yet a prenatal approach also carries inherent risks regarding germline transduction, developmental aberrations and oncogenesis. These concepts are reviewed in detail below.

Vector dose

From birth to adulthood the human body mass increases approximately 20-fold. Therefore, a relatively small amount of GTA will target a higher percentage of cells when introduced early rather than late in life. This is especially advantageous when viral vector systems are used where titers are limiting, such as LV.

Prevention of abnormalities

Genetic mutations can cause a wide spectrum of abnormalities ranging from asymptomatic to lethal. Many pulmonary diseases such as CF will not result in any pathology during prenatal life. For these and many other genetic diseases prenatal gene therapy, even if only providing partial correction, may have a dramatic effect upon disease onset as well as progression.

Tissue characteristics

The relative abundance of stem and progenitor cells in the fetus make the prenatal environment uniquely suitable for gene therapy. Specifically integrating vectors will maintain stable gene expression and provide restitution in a large proportion of the adult tissue. To gain access to an organ such as the lung, a GTA either has to be injected directly into the airways (Carlon et al. 2010, Peebles et al., 2004) or the parenchyma (Henriques-Coelho et al., 2007).

Fetal immunology

In postnatal or adult life, the organism’s defenses against pathogens may be roughly categorized as innate and adaptive immune mechanisms. An additional defense system in fetal life is provided by the maternal immune system, which performs a delicate balancing it has to protect both mother and fetus from microbial infections and at the same time it has to remain tolerant to the fetal semi-allograft (Kammerer et al., 2008). In the fetal lung innate immunity comprises many barriers including immune cells, epithelial cell layers, extracellular matrices and antimicrobial compounds. The fetus is surrounded by amniotic fluid, which not only possesses antibacterial and antifungal properties (Akinbi et al., 2004; King et al., 2007; Yoshio et al., 2003) but also enters the lungs by means of fetal breathing movements. This process washes away the mucus layer, which covers the respiratory epithelium during postnatal life. The developmental immaturity of cells participating in the immune response (Muthukkumar et al., 2000; Takahashi et al., 1995) and the absence of memory cells due to the naivety of the immune system (Adkins et al., 2003) result in an immature adaptive immune response. Tolerance to transgenes after fetal or neonatal transduction has been demonstrated for human factor IX using adenoviral (Waddington et al., 2003), lentiviral (Waddington et al., 2004) or retroviral vectors in mice and dogs (Zhang et al., 2004). In conclusion, although the early adaptive immune system in the fetus appears to be predisposed to tolerance against a transgenic protein, it remains poorly understood compared to the adult immune system. Therefore, careful choice of GTA and rigorous scrutiny of preclinical model systems with attention to differences in immune system development is essential prior to any clinical application.

Adverse effects and risks

In utero gene delivery carries procedural risks that concern the mother as well as the fetus, and include infection, fetal loss, membrane rupture and induction of preterm labor. Other risk factors are specific for fetal gene germline transmission, developmental aberrations and the possibility of insertional mutagenesis. Fetal somatic gene therapy does not attempt to modify the genetic content of the germline. However, the possible induction of genetic changes to the germline is a concern that is central to the field of in utero gene therapy. It is believed that transduction of germline cells in vivo is unlikely to occur, due to the compartmentalization of these primordial germ cells in the gonads, which is completed in humans by the 7^th^ week of gestation. In utero intraperitoneal gene transfer in male sheep (Park et al., 2009; Porada et al., 2005) resulted in transduction of Sertoli cells but not germ cells. Similarly, evidence for LV transduction of a subpopulation of gonadal cells has been observed in female rhesus fetuses after intraperitoneal vector administration (Lee et al., 2005). However, this was not associated with detectable transgene expression and vector has never been found in purified spermatozoa or in the offspring of these animals.

Another possible complication is the occurrence of oncogenesis due to insertional mutagenesis. The risk of insertional mutagenesis is of particular concern after in utero vector application as the fetal system may be specifically sensitive to such events since integrating vectors prefer to insert their genomes into euchromatin (Ciuffi et al., 2006). Recently, a high postnatal incidence of liver tumors was described in mice following prenatal injection of lentiviral vectors derived from the equine infectious anemia virus (EIAV), but not when using a vector derived from HIV (Themis et al., 2005). Somewhat more surprising are the findings of an increase in hepatocellular carcinoma after rAAV injection in newborn mice (Donsante et al., 2001). Later, integrated vector sequences were found by inverse PCR methods in the tumor cells but not in the surrounding tissue (Donsante et al., 2007). In contrast, many other animal studies where rAAV was injected early in life, did not result in oncogenic events (Russell, 2007). The molecular mechanisms underlying these oncogenic events need further elucidation and will ultimately lead to the design of safer vectors and protocols for both pre- and postnatal gene transfer.

Animal experimentation on prenatal gene therapy for the lung

The majority of experiments dealing with fetal gene therapy have been performed in small animal models. The obvious advantages of these include limited ethical and financial constraints, wide availability and low housing demands, well-documented embryogenesis and fetal development, short gestation, large litter size, and in some species the availability of transgenic disease models. As always the mouse has been the irreplaceable model. Yet some aspects of the murine physiology and embryogenesis differ substantially from the human situation. One such example is lung development.

In figure 8 an overview is given of important differences between species regarding the time point of occurrence and duration of the various stages of lung development compared to the human.

Extensive research has been performed in small animals as a lot of genetic disease models exist, e.g. CF specific knock-out or mutant mouse models (Scholte et al., 2004). For the delivery of a therapeutic vehicle encoding a correct copy of an affected or protective gene, different access routes have been described to target the fetal lung in preclinical rodent models, including intratracheal injection (Carlon et al., 2010; Skarsgard et al., 2005), intra-amniotic injection (Boyle et al., 2001; Buckley et al., 2008; Davies et al., 2008; Mitchell et al., 2000), (ultrasound-guided) intrapulmonary injection (Henriques-Coelho et al., 2007; Toelen et al., 2007) and intravenous administration into the yolk sac vessels (Waddington et al., 2003; Waddington et al., 2004) or umbilical vein (Senoo et al., 2000). Ideally an animal model should mimic the disease studied. In the context of CF the mouse model is not the most ideal model for investigating the potential of fetal pulmonary gene therapy as most CF mice mainly develop intestinal symptoms, with only few pulmonary problems. Furthermore airway morphology is significantly different (Grubb et al., 1999). Therefore efforts are being done to induce CF in higher species, such as ferret (Li et al., 2003), sheep (Harris, 1997) and pig (Welsh et al., 2009). Large animal models have the advantage of showing more homology with humans in terms of lung development, lung morphology, duration of pregnancy and fetus size. Furthermore, the in utero surgical procedures performed in sheep (David et al., 2003; Peebles et al., 2004; Porada et al., 2004) and primates (Garrett et al., 2003; Tarantal et al., 2001) are less invasive and therefore more directly applicable to the human fetus.

Conclusion

Progress in both genetic prenatal screening and fetal imaging will further increase the demand for fetal interventions in the near future. The fetal respiratory system is only one target for candidate prenatal interventions. CCAM is a condition which can be easily diagnosed in utero. When fetal hydrops presents prior to viability, fetal therapy seems mandatory. Open fetal surgery has successfully salvaged fetuses with microcystic lesions, while larger cystic lesions have been shunted. Obstructed upper airways can be explored or freed at the time of birth, using the placenta as a heart-lung machine during the so called EXIT procedure. Prenatal imaging can also diagnose congenital diaphragmatic hernia, may rule out frequently associated anomalies and determine prognosis. Fetuses with liver herniation into the thorax and a small lung (LHR < 1.0) have a poor prognosis when managed with standard postnatal therapy. FETO may improve survival from < 10% to above 50%. Outcome can be predicted from preoperative lung size. The procedure carries a significant risk for PPROM and preterm delivery. A randomized trial will have to demonstrate whether prenatal therapy is truly beneficial. The above technique has demonstrated the feasibility of accessing the fetal airways, which can even be punctured to deliver pharmacological agents. It seems therefore logic to consider the potential of fetal pulmonary gene therapy. Presently fetal somatic gene therapy is still a purely experimental approach using animal models as substitute. Clinical trials have shown the necessity of extensive preclinical testing before moving towards human test subjects. Once proven to be safe and reliable it might become an alternative treatment option in selected diseases.